Research Technician IV – Pathology
Description
The Sgourakis laboratory at The Children’s Hospital of Philadelphia and University of Pennsylvania seeks an experienced research assistant to assist in running a busy experimental laboratory, including conducting routine protein biochemistry / molecular immunology experiments. Among other duties, the candidate will have specific responsibilities in generating numerous protein samples for biophysical studies. The candidate will be involved in all aspects of the lab’s NGS workflows, developing new methodologies for these technologies, as well as implementing and troubleshooting NGS protocols. The ideal candidate will:
- Assist in running a busy experimental laboratory, including expressing and purifying proteins, performing molecular assays including molecular cloning, and keeping clear experimental records according to a specific format.
- Communicate complex ideas and concepts to those with limited knowledge and understanding, as well as to peers.
- Have a Bachelor’s degree and at least 2 years of relevant experience or a Master’s degree in Molecular/Cell Biology, Biotechnology, or a related discipline from a leading academic institution.
- Work in a team setting to develop new assays, optimize protocols and troubleshoot issues
Preferred technical skills include a background in protein expression and purification in E.coli and/or insect cell systems (Sf9, S2) and in basic biochemical assays. Additional experience in mammalian cell culture and flow cytometry protocols is desired.
Please send CV and cover letter to Dr. Nik Sgourakis (sgourakisn@chop.edu)
Job Responsibilities
- Independently perform advanced, complex research procedures on experimental samples
- Formulate and perform lab experiments, analyze results, interpret methodology, report data, and maintain appropriate log books and lab notebook
- Modify methods and protocols as required with minimum direction from PI/Sr. Scientist
- Assist in development and troubleshooting of new techniques and ideas
- Prepare lab reagents, monitor and maintain lab inventory
- Supervise, train, and mentor junior and new staff members
- Determine work assignments, schedules, and lab priorities
- Present and discuss work at weekly lab meetings and national symposia
- Assist in writing of grants, journal articles, poster, and oral presentations
Required Education and Experience
- Bachelor’s degree and 1 year of relevant experience, or Master’s degree
- Minimum 2 years of relevant lab experience beyond classroom/internship(s) required
- 24 months advanced laboratory experience
